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. 1998 Nov 10;95(23):13658–13663. doi: 10.1073/pnas.95.23.13658

Figure 5.

Figure 5

c-Src kinase is required for invasin-mediated uptake. (A) Kinase defective Src interferes with bacterial internalization. The amount of bacterial internalization was determined for CEF cells transfected with RCAS derivatives including vector alone (vector) or overexpressing c-Src kinase or kinase-defective Src (srcRF). %UPTAKE refers to the amount of internalization observed relative to that of the vector-transfected control. A representative experiment is shown with error bars indicating the SE for samples prepared in triplicate. (B) Src coexpression blocks FAK stimulation of uptake. Bacterial internalization was determined in CEF cells for vector-transfected cells or cells overexpressing mixtures of FAK or c-src derivatives. %UPTAKE refers to the amount of internalization observed relative to that of the vector-transfected control. Results are the mean of four independent experiments with error bars representing the SE for 13 samples. (C) Cell lysates of RCAS vector transfected and cells overexpressing FAK, c-Src, kinase-defective Src (srcRF), or cooverexpressing FAK and c-Src (FAK/c-Src), FAK and kinase-defective Src (FAK/srcRF) were immunoblotted with anti-FAK and anti-Src antibodies.