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. 1998 Nov 10;95(23):13670–13675. doi: 10.1073/pnas.95.23.13670

Figure 1.

Figure 1

Binary tet-inducible reporter system. A SIN retroviral backbone was used for the reporter virus (Hermes-HRIgfp-hGH) to eliminate transcriptional interference from the viral LTR. The tet-sensitive transactivators (either tTA or rtTA) are provided constitutively from a second retrovirus [Retrotet RTAb(−) or Retrotet RTAb(+)]. Under proper dox conditions, these transactivators bind to the tet-operator sites (O7) fused upstream of the CMV core promoter, inducing transcription of a bicistronic mRNA encoding hGH and GFP from the reporter vector.