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. 1998 Nov 10;95(23):13670–13675. doi: 10.1073/pnas.95.23.13670

Figure 3.

Figure 3

Dose response of binary tet-inducible system. dox dose response of hGH expression (○) and GFP expression (▵) for both RTAb(−) and RTAb(+) systems. hGH and GFP expression of RTAb(−) and RTAb(+) myoblasts cultured in eight different doses of dox were assayed after 3 days under these conditions [RTAb(−) dox doses: 0, 0.0001, 0.0002, 0.0005, 0.001, 0.005, 0.01, and 0.1 μg/ml; RTAb(+): 0, 0.001, 0.01, 0.05, 0.1, 0.5, 1, and 5 μg/ml]. Secreted hGH was measured by radioimmunoassay and normalized to total cell protein. GFP expression was determined by FACS.