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The reduction of bound ARF in multistage reactions. Western blot indicating the amount of ARF and coatomer bound to Golgi membranes as a result of multistage incubations. Lanes 1 and 2 show duplicate, independent reactions carried out in two stages exactly as described for Fig. 1. Lanes 3 and 4 show duplicate results obtained when an “intermediate” incubation (15 min at 37°C), to allow ARF to dissociate, is interposed between stages 1 and 2 in the absence of both ARF and coatomer.
