Figure 9.

Effect of replacing elements of the flgKL class 3 promoter with elements of other class 3 promoters. For each of four class 3 promoters, the −10 and −35 regions alone were substituted into the flgKL class 3 promoter (gray circles). Also, the sequence upstream of the transcription start site through 25 bp upstream of the −35 region for each of the four promoters was used to replace the flgKL promoter upstream of the transcription start site (black circles). The activities of these hybrid promoters were plotted against the total log-odds score for their −10 and −35 regions. A high log-odds score indicates a close match to the consensus sequence. All activities are normalized to the level of transcription from the wildtype flgKL promoter (empty circle).