Table 1.
Salmonella strains used in this study.
| Strain | Genotype | Referencea | |
|---|---|---|---|
| TH437 | LT2 | J. Roth | |
| TH2090 | fliG5101::MudJ | lab collection | |
| TH2142 | hisD9953::MudJ his-9944::MudI | Hughes and Roth29 | |
| TH2534 | flgA5211::MudJ | Gillen and Hughes33 | |
| TH2570 | flgC5215::MudJ | Gillen and Hughes33 | |
| TH2779 | flgM5222::MudJ | Gillen and Hughes33 | |
| TH2856 | fliA5059::Tn10dCm | Karlinsey, et. al.34 | |
| TH2945 | ataA::[P22 Kn9 PfliA(−600)-lacZYA-'9] | M. Chadsey and G. Chilcott | |
| TH3730 | PflhDC5451::Tn10dTc[Δ25] | Karlinsey, et. al.35 | |
| TH4212 | fliS5480::MudK | H. Bonifield | |
| TH4702 | pKD46 (λ-red recombinase plasmid, AmpR, temperature sensitive replication) | Datsenko and Wanner26 | |
| TH4721 | flgK5396::MudJ | Aldridge, et. al.36 | |
| TH5504 | ΔfliA5647::FRT | Aldridge, et. al.27 | |
| TH5794 | PfliD5744::Tn10dTc ΔflgHI958 fljB e,n,x vh2 | lab collection | |
| TH6701 | ΔaraBAD925::tetRA | P. Aldridge | |
| TH7023 | ΔIS200IV::cat(fli-5583) | lab collection | |
| TH7270 | flgJ5964::tetRA(inserted after stop codon) n | ||
| TH7395 | ΔflgM5628::FRT motA5461::MudJ ΔaraBAD923::flgM-FKF ParaB935 | Aldridge, et. al.27 | |
| TH7396 | ΔflgM5628::FRT motA5461::MudJ ΔaraBAD923::flgM-FKF ParaB936 | Aldridge, et. al.27 | |
| TH8006 | pKD46 / ΔaraBAD925::tetRA ΔfliA5647::FRT | ||
| TH8082 | PflhDC5451::Tn10dTc[Δ25] ΔaraBAD943::fliA ΔfliA5647::FRT | ||
| TH8083 | CRR4108[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD943::fliA ΔfliA5647::FRT | ||
| TH8091 | flgA6093::tetRA (inserted after stop codon) | ||
| TH8239 | ΔaraBAD943::fliA ΔfliA5647::FRT | ||
| TH8315 | pKD46 / CRR4108[PflhDC5451::Tn10dTc[ Δ25](TcS)] ΔaraBAD943::fliA ΔfliA5647::FRT flgK5396::MudJ flgJ5964::tetRA | ||
| TH8321 | pKD46 / CRR4108[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD943::fliA ΔfliA5647::FRT fliS5480::MudK fliD5744::Tn10dTc | ||
| TH8922 | fliA5890:Tn10dTc[ Δ25, IS10R(Δ2808–2894)] Δtar-flhD2039 fljBe,n,x vh2 | ||
| TH8925 | ΔaraBAD956::fliA ΔfliA5647::FRT | ||
| TH8926 | ΔaraBAD956::fliA ΔfliA5647::FRT PflhDC5451::Tn10dTc[Δ25] | ||
| TH8927 | ΔaraBAD956::fliA ΔfliA5647::FRT CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] | ||
| TH8928 | ΔaraBAD956::fliA ΔfliA5647::FRT CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ataA::P22[sieA'-Kn6-PfliA(−583 to +1) - lacZYA'-'9] | ||
| TH8929 | ΔaraBAD956::fliA ΔfliA5647::FRT CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] flgK5396::MudJ | ||
| TH8931 | ΔaraBAD956::fliA ΔfliA5647::FRT CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] fliS5480::MudK | ||
| TH8933 | ΔaraBAD956::fliA ΔfliA5647::FRT CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] flgM5222::MudJ | ||
| TH8936 | pKD46 / CRR4107[PflhDC5451::Tn10dTc[ Δ25](TcS)] ΔaraBAD956::fliA ΔfliA5647::FRT flgK5396::MudJ flgJ5964::tetRA | ||
| TH8937 | pKD46 / CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA ΔfliA5647::FRT fliS5480::MudK fliD5744::Tn10dTc | ||
| TH8938 | pKD46 / CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA ΔfliA5647::FRT flgM5222::MudJ flgA6093::tetRA | ||
| TH8939 | pKD46 / CRR4107[PflhDC5451::Tn10dTc[ Δ25](TcS)] ΔaraBAD956::fliA ΔfliA5647::FRT ataA::P22[sieA'-Kn6-PfliA(−583 to +1)-lacZYA'-'9] | ||
| TH9250 | fliA6399::tetRA (replaces −79 to −44 bp from GTG with tetRA) | ||
| TH9252 | pKD46 / CRR4107[PflhDC5451::Tn10dTc[ Δ25](TcS)] ΔaraBAD956::fliA ΔfliA5647::FRT ataA::P22[sieA'-Km6-PfliA(−583 to +1) 6400::tetRA-lacZYA'-'9] | ||
| TH10022 | Δfli-5583::cat(ΔIS200IV) fliA5890::Tn10dTc[ Δ25, IS10R(Δ2808–2894)] Δtar-flhD2039 fljBe,n,xvh2 | ||
| TH10032 | ΔaraBAD956::fliA fliZ6591::MudJ fliA5890::Tn10dTc[ Δ25, IS10R(Δ2808–2894)] | ||
| TH10049 | fliZ6591::MudJ | ||
| TH10132 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA fliZ6591::MudJ ΔfliA5647::FRT | ||
| TH10151 | pKD46 / CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA fliZ6591::MudJ ΔfliA5647::FRT fliA6399::tetRA | ||
| TH10826 | fliA6785::tetRA (inserted −43 bp from GTG) | ||
| TH10896 | pKD46 / CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA fliZ6591::MudJ ΔfliA5647::FRT fliA6785::tetRA | ||
| TH11808 | fliA7226::tetRA (inserted −79 bp from GTG) | ||
| TH12065 | pKD46 / CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA fliZ6591::MudJ ΔfliA5647::FRT fliA7226::tetRA | ||
| TH12359 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA ΔfliA5647::FRT motA5461::MudJ | ||
| TH12906 | Para998::tetRA ΔaraBAD956::fliA ΔfliA5647::FRT | ||
| TH12927 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA fliA5059::Tn10dCm flgC5215::MudJ | ||
| TH12930 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA fliA5059::Tn10dCm flgA5211::MudJ | ||
| TH12933 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA fliA5059::Tn10dCm fliG5101::MudJ | ||
| TH12990 | pKD46 / CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA Para998::tetRA ΔfliA5647::FRT motA5461::MudJ | ||
| TH13035 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA ParaB935(−85 A:T) ΔfliA5647::FRT motA5461::MudJ | ||
| TH13036 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA ParaB936(−41 T:C) ΔfliA5647::FRT motA5461::MudJ | ||
| TH13127 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA ParaB935 ΔfliA5647::FRT flgJ6310::tetRA | ||
| TH13128 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ΔaraBAD956::fliA ParaB936 ΔfliA5647::FRT flgJ6310::tetRA | ||
| TH13161 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ParaB936 ΔaraBAD956::fliA ΔfliA5647::FRT flgK5396::MudJ | ||
| TH13237 | CRR4107[PflhDC5451::Tn10dTc[Δ25](TcS)] ParaB935 ΔaraBAD956::fliA ΔfliA5647::FRT flgK5396::MudJ | ||
a
Strains given no reference were constructed for this study.