Table 2.

Microarray reference/sample ratio ± standard error of microarray clones identifies the methylation status of the selected clones. A ratio greater than one indicates the region is hypomethylated in the test sample relative to the reference sample. A ratio less than one indicates the region is hypermethylated in the test sample relative to the reference sample. These clones were selected to validate the microarray results by using bisulfite sequencing. A significant difference was detected by using ANOVA between the in vivo-produced blastocysts and the other samples (^a - P<0.05, ^b- P<0.01, ^c-P<0.001, ^d -P<0.0001). There was a significant difference between the in vivo-produced blastocysts versus the other samples in 32/60 (53.33%) of the samples. n.d.- no data.

	Sperm	Oocyte	Parth. blast.	SCNT blast.	In vitro blast.	In vivo blast.
B G2	0.960±0.172 c	1.043±0.181 c	0.992±0.203 c	1.748±0.241 b	0.878±0.187 c	21.520±11.047
CC C1	2.055±0.545 b	1.145±0.607	0.354±0.455	0.324±0.293	n.d.	0.375±0.565
EE A11	1.262±0.208 c	0.867±0.201 b	0.560±0.243 b	0.476±0.240 a	1.232±0.199 c	0.173±0.354
EEE D4	1.074±0.191 c	2.825±0.223 b	3.381±0.484 a	2.544±0.710 a	1.463±0.235 b	32.965±20.055
HH A7	0.619±0.427 c	2.033±0.773 a	2.539±2.283 a	0.354±0.677	4.885±1.886 b	0.558±0.673
K D3	0.593±0.214 c	0.411±0.548 a	1.674±0.501	1.432±0.291	3.364	2.806±1.259
L E8	1.033±0.229 a	0.804±0.253	0.485±0.242	0.552±0.242	0.721±0.446	0.464±0.450
O D10	1.105±0.175 d	1.068±0.188 c	1.178±0.214 c	1.546±0.239 b	0.990±0.196 b	6.897±0.966
QQ E4	0.462±0.271	0.179±0.320	3.438±1.776	0.965±0.401	n.d.	1.135±0.716
S E3	0.910±0.290	1.691±0.721	1.235±0.574	0.833±0.318	n.d.	0.859±0.578
WW G4	1.506±0.413 b	2.378±0.943	2.054±1.158	0.218±1.187	3.959±0.976	5.927±2.425
X G2	1.251±0.203 b	1.203±0.185 b	1.240±0.328	2.129±0.313	0.938±0.322 b	3.596±0.692