. Author manuscript; available in PMC: 2009 Feb 1.

Published in final edited form as: Mol Reprod Dev. 2008 Feb;75(2):250–264. doi: 10.1002/mrd.20786

Table 3.

Methylation status of B G2, HH A7, WW G4, and X G2 for in vivo-produced blastocysts analyzed by using microarray and bisulfite sequencing analysis. A.Shown here is the percent cytosine methylation at all the CpG dinucleotides that were analyzed by using bisulfite sequencing in the liver DNA, sperm DNA, and in vivo-produced blastocyst DNA for the regions analyzed by using bisulfite sequencing. B) Bisulfite analysis data and the microarray analysis data are in agreement for 87.5% (7/8) of the samples.

A
	Bisulfite Analysis
CPG clone	Liver	Sperm	In vivo blast.
B G2	0.692	0.024	0.053
HH A7	0.092	0.543	0.313
WW G4	0.341	0.018	0.008
X G2	0.807	0.657	0.667
B
	Sperm (Ref/Sample)		In vivo-produced Blast (Ref/Sample)
CPG clone	Bisulfite	Microarray	Bisulfite	Microarray
B G2	(3.173)	(0.960)	3.078	21.520
HH A7	0.504	0.619	0.757	0.558
WW G4	1.490	1.506	1.505	5.927
X G2	1.778	1.251	1.728	3.596