Table 5.
BLAST analysis of sequenced clones that were hypermethylated in the in vivo-produced blastocysts relative to the liver and their ratios. A significant difference was detected by using ANOVA between the in vivo-produced blastocysts and the other samples (a - P<0.05, b- P<0.01, c-P<0.001, d -P<0.0001). There was a significant difference between the in vivo-produced blastocysts versus the other samples with 129/180 (71.6%) of the samples. n.d.- no data.
| Clone ID | Annotation | Gene | IVP±SE | Oocyte±SE | Sperm±SE | Parth±SE | SCNT±SE | IVF±SE |
|---|---|---|---|---|---|---|---|---|
| AA A1 | NS | 12.490±1.608 | 3.084±0.695 b | 1.184±0.169 d | 1.753±0.370 b | 1.621±0.283 b | 0.730±0.198 c | |
| B G2 | NS | 21.521±11.047 | 1.043±0.181 c | 0.960±0.172 c | 0.992±0.203 c | 1.748±0.241 c | 0.878±0.187 b | |
| BBB A12 | Sus scrofa glutamate decarboxylase 2 (GAD2), mRNA | GAD2 | 15.517±1.937 | 1.259±0.210 d | 1.027±0.175 d | 1.333±0.224 d | 2.515±0.546 d | 2.478±0.340 b |
| BBB H7 | Multiple | 10.564±2.250 | 0.887±0.183 d | 0.887±0.183 d | 1.107±0.197 c | 1.240±0.248 c | 0.735±0.224 b | |
| CCC H7 | Multiple | 3.517±1.258 | 1.795±0.190 | 29.000±0.000 | 2.284±0.440 | 3.594 | 0.591±0.205 | |
| D C10 | NS | 2.676±0.722 | 0.727±0.231 b | 2.187±0.498 | 1.524±0.298 | 1.186±0.433 | 1.469±0.187 a | |
| D D10 | NS | 30.503±13.704 | 0.000±0.000 | 1.282±0.211 c | 2.061±0.245 b | 1.087±0.201 b | 1.484±0.289 c | |
| D D6 | Multiple | 8.786±1.431 | 0.795±0.211 c | 1.607±0.383 c | 1.938±0.391 b | 1.197±0.209 b | 2.543±0.868 c | |
| EEE B7 | Multiple | 58.478±25.479 | 0.818±0.213 c | 1.264±0.216 c | 3.582±0.764 b | 4.234±1.233 b | 0.761±0.207 b | |
| EEE B9 | Multiple | 20.484±31.499 | 0.789±0.169 b | 1.019±0.203 a | 1.287±0.238 a | 0.806±0.211 a | 1.468±0.209 b | |
| EEE E3 | only Bac matches | 15.919±2.731 | 1.255±0.176 d | 2.078±0.305 c | 2.919±0.359 b | 2.231±0.245 b | 0.934±0.180 c | |
| F E10 | Multiple | 24.933±27.214 | 1.082±0.173 b | 1.043±0.198 b | 1.425±0.251 a | 1.043±0.198 a | 1.125±0.187 b | |
| F F10 | NS | 4.001±0.781 | 1.287±0.188 b | 2.041±0.298 | 2.057±0.391 | 1.940±0.479 | 1.953±0.201 | |
| FF G1 | only Bac matches | 6.205±5.827 | 0.835±0.240 a | 1.986±0.314 | 2.356±0.373 | 2.604±1.120 | 0.862±0.296 | |
| G G10 | PREDICTED: Canis familiaris similar-DEAD (Asp-Glu-Ala-Asp) box | DDX10 | 1.745±1.198 | 0.836±0.245 a | 1.371±1.820 | 1.465±0.296 | 0.000±0.000 | 1.587±0.676 |
| GGG D4 | WNT8B gene | WNT8B | 0.750±0.759 | 1.735±1.364 | 4.797±2.769 a | 1.293±0.332 | 7.000±0.000 | 1.769±0.232 a |
| II H10 | Multiple | 5.443±1.633 | 1.248±0.193 b | 1.806±0.496 | 2.961±0.471 | 1.875±1.612 | 0.866±0.185 | |
| III D1 | Multiple | 27.565±10.203 | 0.793±0.173 d | 1.334±0.207 c | 1.809±0.390 b | 0.690±0.200 b | 0.793±0.237 d | |
| JJ B10 | Bos taurus similar-Homeobox Protein SIX6 (Sine oculis homeobox homolog 6) | SIX6 | 22.006±8.660 | 0.971±0.182 c | 0.995±0.198 c | 1.162±0.242 c | 0.729±0.217 c | 0.901±0.179 c |
| JJ D12 | H.sapiens CpG island DNA | 19.756±9.884 | 0.789±0.172 c | 1.250±0.202 b | 1.468±0.421 b | 0.852±0.185 b | 0.804±0.183 c | |
| JJ E10 | NS | 30.306±19.817 | 0.801±0.171 c | 1.158±0.231 b | 1.565±0.277 b | 0.917±0.223 b | 1.262±0.185 b | |
| K G10 | Human cyclic AMP transcriptional regulator binding protein (CRE-BP1) | ATF2 | 2.837±0.464 | 1.005±0.171 c | 1.034±0.211 b | 1.353±0.241 a | 0.604±0.509 a | 1.370±0.183 b |
| LL E4 | NS | 11.712±1.302 | 1.196±0.169 d | 1.187±0.221 d | 1.610±0.245 d | 1.176±0.190 d | 0.647±0.187 d | |
| NN G9 | NS | 2.848±0.427 | 1.709±0.204 a | 1.221±0.553 | 2.106±0.820 | 1.522±0.315 | 0.786±0.183 a | |
| O D12 | Multiple | 26.245±27.961 | 0.993±0.257 b | 1.191±0.201 b | 1.244±0.257 a | 1.152±0.181 a | 1.008±0.263 b | |
| P F6 | Bos taurus similar-protoporphyrinogen oxidase, Last enzyme of heme synthesis | PPOX | 2.328±0.674 | 0.873±0.347 a | 2.094±0.586 | 1.582±0.372 | 1.228±0.818 | 0.770±0.204 |
| P H5 | PREDICTED: Bos Taurussimilar-zinc finger, CSL domain | ZCSL2 | 34.769±19.815 | 2.245±0.778 b | 1.042±0.347 c | 1.124±0.371 b | 1.135±0.272 c | n.d. |
| QQ A6 | Multiple | 29.106±36.958 | 0.926±0.169 b | 1.133±0.205 b | 1.632±0.247 a | 0.943±0.205 a | 1.014±0.185 b | |
| RR G5 | H. sapiens genes for histones H2B.1 and H2A | HIST2H2BE | 12.783±5.020 | 1.007±0.198 c | 2.118±0.446 a | 2.115±0.370 a | 0.902±0.292 a | 1.526±0.381 b |
| UU C10 | NS | 1.458±0.862 | 0.697±0.258 | 2.993±0.859 | 1.066±0.325 | 0.010±0.000 | 0.882±0.182 b | |
| UU H3 | NS | 1.723±0.653 | 1.096±0.210 | 0.838±0.251 b | 1.767±0.350 | 1.412±0.466 | n.d. | |
| X F12 | Homo sapiens prostate antigen PARIS-1 mRNA, complete cds | TBC1D2 | 8.198±1.664 | 0.847±0.185 d | 1.431±0.325 b | 1.554±0.246 b | 0.723±0.204 b | 1.203±0.185 c |
| X G2 | NS | 3.596±0.692 | 1.251±0.203 b | 1.240±0.328 a | 2.129±0.313 | 0.938±0.322 | 1.042±0.210 b | |
| XX H10 | NS | 15.403±5.999 | 0.979±0.178 c | 1.450±0.245 b | 2.382±0.781 a | 0.961±0.213 a | 1.166±0.181 b | |
| XX H12 | Homo sapiens splicing factor 3a, subunit 3, 60kDa (SF3A3), mRNA | SF3A3 | 30.199±16.716 | 1.102±0.169 c | 1.089±0.213 b | 1.642±0.405 b | 0.775±0.194 b | 1.016±0.187 c |
| Z D3 | NS | 22.118±9.730 | 0.877±0.189 c | 1.226±0.205 c | 1.485±0.253 b | 0.812±0.255 b | n.d. |