Table 6.
BLAST analysis of sequenced clones that were hypermethylated in the in vivo-produced blastocysts relative to the liver and their ratios. A significant difference was detected by using ANOVA between the in vivo-produced blastocysts and the other samples (a - P<0.05, b- P<0.01, c-P<0.001, d -P<0.0001). There was a significant difference between the in vivo-produced blastocysts versus the other samples with 97/160 (58.78%) of the samples. n.d.- no data.
| Clone ID | Annotation | Gene | IVP blast±SE | Oocyte±SE | Sperm±SE | Parth blast±SE | SCNT blast±SE | IVF blast±SE |
|---|---|---|---|---|---|---|---|---|
| BLUE E3 | NS | 0.206±0.371 | 1.323±0.456 b | 1.904±0.388 c | 0.343±0.292 | 0.237±0.285 | 1.060±0.696 a | |
| CC C1 | NS | 0.375±0.565 | 1.450±0.607 | 2.055±0.545 b | 0.345±0.455 | 0.324±0.293 | n.d. | |
| CCC B6 | Multiple | 0.632±0.898 | 1.386±0.459 | 0.782±0.374 | 1.345±0.585 | 0.808±0.297 | 0.723±0.000 | |
| EE A11 | Membrane bound O-acyltransferase domain containing 5 | MBOA T5 | 0.173±0.354 | 0.876±0.201 b | 1.262±0.208 c | 0.560±0.243 b | 0.476±0.241 a | 1.232±0.199 c |
| EE A12 | NS | 0.146±0.361 | 1.072±0.207 b | 1.469±0.277 b | 0.315±0.245 | 0.264±0.248 | 0.935±0.386 a | |
| EE H2 | Multiple | 0.173±0.409 | 1.131±0.284 c | 1.608±0.580 b | 0.338±0.355 | 0.334±0.235 a | 0.966±0.301 c | |
| EE H8 | Homo sapiens arylhydrocarbon receptor nuclear translocator | ARNT | 0.241±0.405 | 1.250±0.228 b | 1.331±0.219 c | 0.548±0.270 a | 0.471±0.247 | 0.963±0.443 b |
| FF E4 | Multiple immune components | 0.441±0.353 | 0.743±0.184 b | 0.888±0.218 b | 0.872±0.210 b | 0.992±0.244 b | 0.864±0.181 a | |
| G B8 | Canis familiaris simitCoatomer zeta-1 subunit | COPZ 1 | 0.232±0.412 | 0.818±0.215 b | 0.299±0.830 | 0.379±0.308 | 0.262±0.241 | n.d |
| N E12 | Homo sapiens serine/threonine protein kinase Kp78 (ribosomal) | MARK 3 | 1.335±0.465 | 0.974±0.252 | 1.001±0.272 | 1.512±0.285 | 1.144±0.377 | 0.816±0.334 |
| HH A7 | NS | 0.558±0.673 | 2.033±0.773 a | 0.619±0.427 | 2.539±2.283 a | 0.354±0.677 | 4.885±1.886 b | |
| L E8 | CpG Island plus others | 0.464±0.450 | 0.804±0.253 | 1.033±0.229 a | 0.485±0.242 | 0.552±0.242 | 0.721±0.446 | |
| LL D3 | NS | 0.555±0.718 | 0.893±0.687 | 1.344±0.777 | 0.180±0.338 | 0.842±0.000 | n.d. | |
| NN F4 | Mus musculus RIKEN cDNA 2810429O05 gene | 0.349±0.581 | 0.000±0.000 | 0.000±0.000 | 0.190±0.535 | 0.401±0.303 | n.d. | |
| PINK E2 | NS | 0.027±0.399 | 1.578±0.329 c | 1.642±0.185 d | 0.379±0.285 a | 0.216±0.259 a | 0.788±0.340 c | |
| PINK E9 | NS | 0.041±0.349 | 0.807±0.207 c | 0.955±0.174 d | 0.546±0.238 c | 0.366±0.251 b | 0.804±0.190 d | |
| PINK E10 | Multiple | 0.050±0.399 | 0.829±0.183 d | 0.932±0.170 d | 0.587±0.229 c | 0.500±0.243 c | 0.752±0.246 c | |
| PP C2 | NS | 0.091±0.405 | 0.972±0.239 b | 0.964±0.261 c | 0.186±0.320 | 0.193±0.238 | 0.961±0.000 a | |
| PP D6 | NS | 0.208±0.406 | 1.110±0.179 b | 1.188±0.170 c | 0.884±0.213 b | 0.695±0.237 a | 1.478±0.209 b | |
| PP E2 | Multiple | 0.135±0.401 | 1.205±0.428 b | 1.268±0.187 d | 0.567±0.327 | 0.315±0.243 a | 1.187±0.319 c | |
| PP E4 | PREDICTED: Bos taurus similarto malignant T cell amplified sequence 1 | MCTS 1 | 0.503±0.430 | 1.493±0.182 b | 1.044±0.173 a | 0.846±0.235 | 0.667±0.260 | 0.908±0.223 |
| PP E5 | PREDICTED: Canis familiaris Similar to Methyltransferase-like | 0.183±0.353 | 1.422±0.187 c | 1.091±0.172 c | 0.770±0.287 a | 0.658±0.250 a | 1.566±0.382 b | |
| PP E6 | PREDICTED: Bos taurus similar to Paired box protein Pax-3 | PAX3 | 0.134±0.403 | 1.774±0.184 c | 1.225±0.169 c | 0.686±0.223 a | 0.583±0.245 a | 1.271±0.213 b |
| PP G1 | NS | 0.118±0.409 | 0.409±0.254 c | 0.798±0.188 d | 0.402±0.308 a | 0.245±0.238 a | 1.095±0.000 b | |
| PP H6 | Homo sapiens FRG1 (FRG1) gene, complete cds (multiple) | FRG1 | 0.104±0.351 | 1.379±0.255 c | 1.131±0.169 c | 0.911±0.202 c | 0.876±0.237 b | 1.314±0.190 c |
| Q A2 | Homo sapiens serine/threonine protein kinase Kp78 (ribosomal) | MARK 3 | 0.268±0.417 | 0.768±0.242 a | 0.607±0.177 a | 0.556±0.210 | 0.498±0.239 | 0.632±0.248 |
| Q H5 | PREDICTED: Bos taurus similar to Forkhead box protein J2 | FOXJ2 | 0.067±0.399 | 0.961±0.220 c | 1.092±0.225 c | 0.425±0.231 c | 0.298±0.235 b | 1.299±0.699 c |
| QQ D3 | NS | 0.642±0.366 | 0.740±0.258 | 0.632±0.176 | 0.738±0.234 | 0.663±0.234 | 2.111±0.912 a | |
| T A6 | NS | 0.217±0.890 | 1.347±0.000 | 1.485±0.873 | 0.772±0.000 | 1.206±0.781 | n.d. | |
| TT G8 | 790G17 on chromosome 1q21.1-21.3 | 0.440±0.661 | 0.737±0.613 | 3.954±0.000 a | 0.289±0.523 | 0.235±0.511 | n.d. | |
| W E3 | NS | 0.147±0.405 | 1.470±0.262 c | 1.134±0.229 c | 0.444±0.285 a | 0.322±0.241 a | 2.809±0.000 b | |
| W F1 | NS | 0.038±0.349 | 1.162±0.219 c | 1.147±0.178 c | 0.760±0.250 c | 0.340±0.236 b | 1.990±0.191 d |