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. 2008 Apr 24;149(8):3860–3869. doi: 10.1210/en.2008-0184

Figure 7.

Figure 7

IHC lamprey GnRH-II-like ir in the hypothalamus (hyp) of adult sea lampreys. Transverse (A) and sagittal (C) sections were stained with antilamprey GnRH-II, which was previously absorbed with a mixture of synthetic lamprey GnRH-I and -III. The areas outlined by the rectangle (A and C) are enlarged and shown in B, D, and E, respectively. Note lamprey GnRH-II-positive cell bodies in the preoptic nucleus (PON) and fibers in the neurohypophysis (NH). OC, Optic chiasm; PI, pars intermedia; POR, preoptic recess; PPD, proximal pars distalis; RPD, rostral pars distalis; III, third ventricle. Scale bars (A and C), 200 μm (A, ×50; C, ×40); B, D, and E, 20 μm (B, ×390; D, ×360; E, ×450).