Figure 3.
Immunocytochemistry of ovary sections showing various levels of AS-A in the corpus luteum during pseudopregnancy and luteolysis. Panels A and C (magnified in panels B and D), Ovary sections during corpus luteum development of pseudopregnant mice (d 4 and 8 in the “pseudopregnancy” experiment, respectively). Panels E and F, An ovary section during luteolysis (experimental d 13, see progesterone levels in Fig. 2A for indication of functional luteolysis). Lower panels (B, D, and F) show magnified areas of the top panels (A, C, and E, respectively). Note that the intensity of the AS-A staining in the corpus luteum was in the following order: d 13 > d 8 > d 4. On experimental d 13, a minor population of the corpora lutea stained for AS-A with less intensity (arrow in panel E). Data shown are representative of duplicate experiments.