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Temporal profile of D1 and NAG activity at different stages of development and functionality of EPI. A, Optimal conditions for D1 activity were used (see Materials and Methods). B, NAG was measured by a colorimetric test. The data were analyzed with a one-way ANOVA, and differences between means were evaluated by the Tukey test. Different letters indicate significant differences between groups (P ≤ 0.05, n = 6).
