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. 2008 May 8;149(8):3870–3880. doi: 10.1210/en.2007-1506

Figure 3.

Figure 3

Effect of icv PTP1B-ASO on leptin-induced satiety and leptin signaling in hypothalamus of control and DIO rats. A, leptin was icv infused, and 12-h food intake was measured in control and DIO rats treated with PTP1B-ASO or PTP1B-sense or in pair-fed rats. B, Immunoprecipitation (IP) with anti-Jak-2 and immunoblotting (IB) with anti-pY antibodies. C, IP with anti-Jak-2 and IB with anti-PTP1B. D, IP with anti-ObR and IB with anti-pY. E, IP with anti-Stat3 and IB with anti- pY. F, IP with anti-Jak-2 and IB with anti-pY. G, IP with anti-Stat3 and IB with anti-pY. Specific bands were densitometrically quantified. Data are means ± sem of four independent experiments, i.e. four different cohorts of control rats or DIO rats. The comparisons between the groups were performed by using the same time point in the respective group: *, P < 0.01 PTP1B-ASO vs. PTP1B sense; #, P < 0.05 PTP1B-sense vs. PTP1B sense; &, P < 0.05 pair-fed vs. PTP1B-ASO; $, P < 0.05 pair-fed vs. PTP1B-sense.