Figure 1.

Direct stimulatory effect of Kp on GnRH release from MBH explants from male mice. After 1-h acclimatization, MBH explants were exposed to different concentrations of Kp10 (0, 0.5, 5, 50, and 500 nm for wild type, and 0 and 500 nm for GPR54−/−) for 1 h and then challenged with 60 mm KCl for 1 h. Columns represent the mean (± sem) of the GnRH released into the culture medium after Kp10 stimulation. Inset shows responses to 60 mm KCl. ***, P < 0.001 compared with all other treatments on both wild type and GPR54−/−. One-way ANOVA followed by the Student-Newman-Keuls multiple range test. The number of animals used per conditions is indicated at the bottom of each column.