Figure 3.

Inhibition of cell proliferation persists on removal of curcumin. A, MMQ cells were treated with vehicle or 50 μm curcumin for 4 d. Cell proliferation was assessed by the MTT assay as described in Materials and Methods (d 0). Medium was then replaced with growth medium containing 10% FBS. Cell proliferation was measured using the MTT assay after 1, 2, 3, and 4 d of culture. Data were expressed as OD and is the mean ± sem of three to six independent determinations. Results are from a single experiment and are representative of three independent experiments yielding similar results. *, Significant differences (P < 0.001) from corresponding day control values; **, significant differences (P < 0.005) from curcumin-treated d 0; ***, significant differences (P < 0.001) from vehicle-treated d 0. B, Mean ± sem values from the three experiments in A were expressed as percent inhibition of cell proliferation relative to control at each day (curcumin treated vs. control).