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. 2008 May 1;149(8):3909–3919. doi: 10.1210/en.2008-0286

Figure 2.

Figure 2

Autocrine hGH enhances anchorage-independent growth in RL95-2 cells. A, Soft agar colony formation of RL95-2-vector and RL95-2-hGH cells in 10% FBS medium. Colonies were visualized under ×150 magnification. B, Growth curve of RL95-2-vector and RL95-2-hGH cells in suspension culture in 10% FBS medium. C, Effect of autocrine hGH on colony formation in growth factor (GF) or growth factor-reduced (GFR) Matrigel. Number of colonies indicates the total numbers of colonies with disrupted morphology and filled lumina. Images were captured under ×400 magnification. D, RL95-2-vector and RL95-2-hGH cell foci formation in 10% FBS medium. **, P < 0.001.