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. 2008 May 1;149(8):3909–3919. doi: 10.1210/en.2008-0286

Figure 6.

Figure 6

The hGH receptor antagonist, B2036, reduces cell viability, total cell number, cell cycle progression, and cell survival in wild-type RL95-2 cells. A and B, The effect of B2036 on the viability of RL95-2 cells was evaluated by MTT assay in 10% FBS (A) and serum-deficient (0.2% serum) (B) conditions. C and D, The effect of B2036 on the growth of RL95-2 cells was evaluated by total cell number in 10% FBS (C) and serum-deficient (0.2% serum) (D) conditions. E, The effect of B2036 on RL95-2 cell cycle progression was assessed by nuclear incorporation of BrdU in 10% FBS and serum-free medium. F, The effect of treatment of RL95-2 and RL95-2-hGH cells with B2036 on apoptotic cell death induced by serum withdrawal over 48 h was assessed by TUNEL assay in serum-free medium. **, P < 0.001.