Figure 2.

Evaluation of apoE gene promoter sequences responsive to TNFα in adipocytes. A, The reporter constructs used to evaluate apoE gene elements responsive to TNFα are shown. Base pairs are numbered relative to the transcription start site, and en represents inclusion of the 620-bp ME1. The hatched box represents the first apoE exon. B, 3T3-L1 adipocytes were transfected with the indicated apoE promoter-luciferase constructs. TNFα (5 ng/ml) was added for 24 h in complete growth medium before harvesting cells for measurement of luciferase and β-galactosidase activities. *, P < 0.01 for comparing TNFα-treated to untreated control. Results shown are mean ± sd from triplicate samples. C, Percent change in relative luciferase units (RLU) after treatment with TNFα is shown for each construct after normalization to the untreated control. *, P < 0.01 for comparing TNFα-treated to untreated control. The results shown are mean ± sd from three independent experiments, each performed using triplicate samples.