Table 5.
QRT-PCR primer sequences for EMT gene expression investigation and cDNA microarray validationa
| Gene | Primer sequence (5′-3′) | Size (bp) | Function | Accession No. |
|---|---|---|---|---|
| GAPDH | F-TGCACCACCAACTGCTTAGC | 87 | Housekeeper | 33 |
| R-GGCATGGACTGTGGTCATGAG | ||||
| SDHA | F-TGGGAACAAGAGGGCATCTG | 86 | Housekeeper | 33 |
| R-CCACCACTGCATCAAATTCATG | ||||
| TGFBR2 | F-GCAGGTGGGAACTGCAAGAT | 76 | EMT | 34 |
| R-GAAGGACTCAACATTCTCCAAATTC | ||||
| S100A4 | F-AACTAAAGGAGCTGCTGACCC | 118 | EMT | BX470102, |
| R-AAGTCCACCTCGTTGTCCC | NM_002961.2 | |||
| SMAD2 | F-CCATCGAAAAGGATTGCC | 98 | EMT | AF027964, |
| R-ATTCGCAGTTTTCAATTGCC | NM_005901.3 | |||
| BMPR2 | F-ACTGGAAATAGACTGGTGCG | 94 | MET | AC009960, |
| R-CTTCTAGCACTTCTGGTGCC | NM_001204.5 | |||
| TGFB1 | F-GCCGCTGCCCATCGTGTACTA | 193 | EMT | BC001180, |
| R-GGCTTGGGCACGGGTGTCC | NM_000660.3 | |||
| BMP7 | F-GATGCAGCGCGAGATCCTC | 170 | MET | AF210054, |
| R-CGGCCTTGTAGGGGTAGGAG | NM_001719.1 | |||
| MMP2 | F-CCCCCAAAACGGACAAAG | 170 | EMT | AY738117, |
| R-CCGCATGGTCTCGATGGTAT | NM_004530.2 | |||
| BCAT | F-CGGAGACGGAGGAAGGTCTGA | 149 | MET | AY463360, |
| R-CACGCTGGATTTTCAAAACAGTTG | NM_002961.2 | |||
| RGPS3 | F-TACCCTCGCTTTCTCCGTTCT | 216 | Array validate | AL359455, |
| R-CCTCGCTTCCGTATGTCTATCC | NM_144488.4 | |||
| IGF2 | F-GGCCCTCCTGGAGACGTACTGT | 98 | Validation and EMT | AF517226, |
| R-CGGGGTATCTGGGGAAGTTGTC | NM_001042377.1 | |||
| COL1A2 | F-ACAGGGTGCTCGTGGTTTC | 93 | Validation and EMT | M21671, |
| R-GGCTGTCCCTTCAATCCATC | NM_000089.3 | |||
| SNAIL2 | F-ATTCGGACCCACACATTACC | 160 | EMT | AF084243, |
| /SLUG | R-GAGCCCTCAGATTTGACCT | NM_003068.3 |
a
BMP7, bone morphogenic protein 7; BMPR2, bone morphogenic protein receptor 2; COL1A2, collagen 1, α 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RGPS3, regulator of G-protein signaling 3; SDHA, succinate dehydrogenase complex, subunit A; SNAIL2/SLUG, snail homolog 2 (Drosophila); TGFB1, transforming growth factor-β1; TGFBR2, transforming growth factor-β receptor 2.