Table 3.
Real-time PCR primersa
| Gene | GenBank Accession No. | Primer Sequences | Amplicon Position |
|---|---|---|---|
| EGR1 | AA956793 | Forward: AAGGCCGAGATGCAATTGAT | 271 to 349 |
| Reverse: TGTCCATGGTGGGTGAGTGA | |||
| TIMP1 | AA957593 | Forward: TCAGAGCCCATGAGGATCTGA | 184 to 246 |
| Reverse: CCCTTGCAAACTGGAGAGTGA | |||
| Col IVα1 | AA964281 | Forward: GGAGCGCCATTTGGAGTGT | 48 to 126 |
| Reverse: TTCGTTGGCCTCTGTTTGCT | |||
| GPSP2 | NM_053453 | Forward: ATTGATTCAATGTTGGGTAGTGAATC | 777 to 855 |
| Reverse: TCACACAGCAAGGGAAACTTCTC | |||
| IGFBP5 | AI070577 | Forward: CTTTAATCCTTGCAATTGGGACTT | 144 to 223 |
| Reverse: CTGTCCTCCAAACACTTGCAAA | |||
| MM2 | NM_031054 | Forward: AAGTGGGACAAGAACCAGATCAC | 825 to 903 |
| Reverse: AAGCATCATCCACGGTTTCAG | |||
| Myl9 | XM_215905 | Forward: CAAGGCCAAGACCACCAAGA | 432 to 510 |
| Reverse: CTGGATCTGGGACTGGTCAAA | |||
| C4 | AY149995 | Forward: TGGATGGTGCCAAGGAGTATC | 1718 to 1796 |
| Reverse: AGTGCCACCAGGGCTTCAG |
a
See legend of Figure 2 for definition of gene abbreviations.