Table 1.
Parameter | Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7 | Group 8 | Group 9 | Group 10 |
---|---|---|---|---|---|---|---|---|---|---|
Mouse strain | C57BL6(wild-type) | LDLR−/− | LDLR−/− | LDLR−/− | LDLR−/− | LDLR−/− | LDLR−/− | LDLR−/− | LDLR−/− | LDLR−/− |
Diet | Regular chow | Regular chow | High fat | Fat | Fat | Fat | Fat | Fat | Fat | Fat |
Surgery | Sham | Sham | Sham | CKD | CKD | CKD | CKD | CKD | CKD | CKD |
Weeks postnatal | 28 | 28 | 28 | 22 | 28 | 28 | 28 | 28 | 28 | 28 |
Treatment | Vehicle | Vehicle | Vehicle | Vehicle | Vehicle | Calcitriol 10 ng/kg | Calcitriol 20 ng/kg | Paricalcitol 50 ng/kg | Paricalcitol 100 ng/kg | Paricalcitol 400 ng/kg |
n | 7 | 7 | 7 | 6 | 7 | 6 | 6 | 10 | 8 | 9 |
Presurgical weight | 18.983 ± 1.010 | 19.314 ± 0.640 | 19.400 ± 0.620 | 21.740 ± 0.750 | 21.500 ± 1.890 | 22.120 ± 2.180 | 21.400 ± 0.835 | 21.020 ± 0.676 | 21.988 ± 1.089 | 22.378 ± 0.691 |
Weight at killing | 21.583 ± 0.759 | 20.580 ± 0.500 | 25.044 ± 1.640 | 21.640 ± 0.950 | 21.067 ± 1.800 | 22.600 ± 1.860 | 24.383 ± 1.807 | 22.580 ± 1.039 | 22.938 ± 1.327 | 24.933 ± 1.205 |
BUN (mg/dl) | 20.960 ± 2.896 | 27.660 ± 4.640 | 18.700 ± 1.660 | 61.200 ± 14.460a | 37.420 ± 3.740a | 54.000 ± 10.000a | 46.400 ± 3.280a | 38.140 ± 4.890a | 49.660 ± 2.690a | 38.710 ± 5.720a |
Glucose (mg/dl)b | 148.410 ± 12.780 | 233.500 ± 20.790 | 155.900 ± 24.480 | 124.800 ± 16.850 | 165.430 ± 14.700 | 207.870 ± 41.260 | 181.400 ± 25.880 | 184.430 ± 26.240 | 174.500 ± 32.910 | 288.140 ± 52.790 |
Cholesterol (mg/dl) | 52.360 ± 4.600 | 199.00 ± 6.800 | 658.900 ± 78.000c | 556.000 ± 70.000c | 702.500 ± 72.00c | 466.620 ± 50.600c | 790.800 ± 167.550c | 814.000 ± 90.720c | 593.500 ± 81.590c | 1213.2008 ± 143.550c |
PTH (pg/ml) | 19.100 ± 5.300 | 25.630 ± 6.300 | 23.510 ± 13.360 | 743.520 ± 186.000d | 444.050 ± 98.000d | 74.600 ± 8.300e | 44.320 ± 9.600e | 83.710 ± 18.800e | 60.340 ± 19.200e | 16.200 ± 2.700e |
All of the CKD groups had significantly higher BUN at killing compared with wild-type or high-fat sham (P < 0.02).
There was no statistical difference among the glucose values.
All the high fat–fed groups had significantly higher cholesterol compared with wild-type (P < 0.001).
The PTH values were higher for the CKD groups compared with wild-type (P < 0.001).
There was a dosage-dependent decrease in PTH compared with the CKD 28-wk vehicle group (untreated; P < 0.001).