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Primer sequences
| Gene | Primer | Sequence(5′-3′) | Product Length (bp) | Temperature (°C) |
|---|---|---|---|---|
| Osterix | Forward | TCCCTTCTCAAGCACCAATGGACT | 230 | 60 |
| Reverse | AGCTGTGAATGGGCTTCTTCCTCA | |||
| GAPDH | Forward | TGTTCCAGTATGACTCCACTCACG | 170 | 60 |
| Reverse | GAAGACACCAGTAGACTCCACGACA | |||
| CBFA1 | Forward | TGCAAGAAGGCTCTGGCGT | 200 | 60 |
| Reverse | CGCTGAAGAGGCTGTTTGACG | |||
| OC | Forward | TCATGTCCAAGCAGGAGGGCAATA | 292 | 58 |
| Reverse | TGACATCCATACTTGCAGGGCAGA | |||
| MSX2 | Forward | ACCACGTCCCAGCTTCTAGC | 183 | 60 |
| Reverse | GCTCTGCGATGGAGAGGTACTG |
