Table 2.

PCR primers

α-globin (full length, 429 bp)

forward: 5′-ATG GTG CTC TCT GCA GAT GA-3′

reverse: 5′-TTA ACG GTA CTT GGA GGT CA-3′

β-globin (full length, 444 bp)

forward: 5′-ATG GTG CAC CTA ACT GAT GCT G-3′

reverse: 5′-TTA GTG GTA CTT GTG AGC CA-3′

α-globin (335 bp)

forward: 5′-CTC TCT GGG GAA GAC AAA AGC AAC-3′

reverse: 5′-GGT GGC TAG CCA AGG TCA CCA GCA-3′

β-globin (416 bp)

forward: 5′-TGA ACC CTG ATG ATG TTG GTG GCG AGG-3′

reverse: 5′-AAG ACA AGA GCA GGA AAA GAG GTT TAG-3′

AE1 (467 bp)

forward: 5′-TGG CTG CTG TCA TCT TCA TCT AC-3′

reverse: 5′-TTT GGG CTT CAT CAC AAC AGG-3′

β-actin (306 bp)

forward: 5′-CTT TCT ACA ATG AGC TGC GTG-3′

reverse: 5′-TCA TGA GGT AGT CTG TCA GG-3′