Figure 11.

VEGF-A is significantly decreased by rapamycin treatment starting 14 d after anti-GBM GN. (A) Thirty-five days after induction of anti-GBM GN, mice were evaluated for their mRNA expression of VEGF-A, nephrin-1, and PECAM-1 in kidneys of rapamycin- (□; n = 8) or vehicle-treated mice (▪; n = 8). Mice treated with rapamycin starting on day 14 after induction of anti-GBM GN displayed significantly decreased expression of VEGF-A and PECAM-1 but equal expression of nephrin-1. (B) Protein expression of VEGF was evaluated in kidneys of rapamycin- and vehicle-treated mice 35 d after induction of anti-GBM GN. A representative Western blot from three independent experiments is shown.