Figure 3.

Rapamycin started on the day of immunization inhibits the infiltration of interstitial CD4⁺ T cells and macrophages. The infiltration of inflammatory cells was analyzed by immunohistochemistry. Kidneys were stained for CD4⁺ T cells and macrophages 14 and 21 d after induction of anti-GBM nephritis in mice treated with rapamycin (□; n = 8 per time point) or vehicle (▪; n = 7 per time point). CD4⁺ T cell infiltration was significantly decreased 14 and 21 d after anti-GBM injection. Interstitial accumulation of F4/80⁺ cells was significantly diminished in rapamycin-treated mice on day 14 and 21 after induction of disease. *P < 0.05.