Figure 5.

The proliferation and production of IFN-γ, TNF-α, and IL-10 of spleen cell cultures is significantly reduced after rapamycin treatment starting on the day of immunization. On day 14 after anti-GBM injection, 1 × 10⁷ splenocytes from animals treated with either rapamycin (□; n = 8) or vehicle (▪; n = 7) were seeded in 24-well plates and stimulated with LPS (100 ng/ml) or PHA (20 mg/ml) for 16 h. (A and B) Proliferation of LPS- and PHA-stimulated splenocytes (A) and cytokine production of PHA-stimulated splenocytes (B) was determined. Proliferation and cytokine production were significantly inhibited by rapamycin treatment. *P < 0.05.