Figure 9.

Rapamycin treatment started 14 d after induction of anti-GBM GN increases the infiltration of interstitial CD4⁺ T cells and macrophages. The infiltration of inflammatory cells was analyzed by immunohistochemistry. Staining for CD4⁺ and macrophages was performed 35 d after induction of anti-GBM nephritis in mice treated with rapamycin (□; n = 8) or vehicle (▪; n = 8). Interstitial accumulation of CD4⁺ T cells and F4/80⁺ cells was significantly increased in rapamycin-treated mice 35 d after induction of disease. *P < 0.05.