Table 1.
Systemic inflammatory response of mice from groups 1 and 2a
| Characteristics of Mice | Group 1
|
Group 2
|
||
|---|---|---|---|---|
| Vehicle | Rapamycin | Vehicle | Rapamycin | |
| Cytokine expression in LN | ||||
| IFN-γ | 53.7 ± 9.2 | 14.9 ± 3.8b | 36.8 ± 7.9 | 22.0 ± 5.5 |
| TNF-α | 4.2 ± 0.5 | 3.0 ± 0.4 | 5.1 ± 1.0 | 5.9 ± 0.8 |
| IL-6 | 23.6 ± 18.9 | 1.5 ± 0.4 | 20.8 ± 5.6 | 21.9 ± 6.8 |
| IL-10 | 3.3 ± 0.3 | 2.9 ± 0.4 | 6.9 ± 1.2 | 6.0 ± 0.8 |
| IL-17 | 10.1 ± 1.3 | 4.6 ± 1.3b | 13.7 ± 3.5 | 15.1 ± 4.0 |
| FoxP3 expression in LN | 5.7 ± 1.1 | 4.9 ± 0.7 | 7.2 ± 2.7 | 4.8 ± 1.2 |
| Splenocyte proliferation | ||||
| proliferation to LPS (OD 450) | 0.50 ± 0.00 | 0.22 ± 0.00b | 1.81 ± 0.10 | 1.92 ± 0.20 |
| proliferation to PHA (OD 450) | 0.48 ± 0.00 | 0.21 ± 0.00b | 2.12 ± 0.20 | 2.00 ± 0.20 |
| B cell response | ||||
| rabbit anti-mouse IgG (OD 450) | 0.73 ± 0.10 | 0.34 ± 0.10b | 0.91 ± 0.10 | 0.73 ± 0.10 |
| Deposition of rabbit IgG (titer) | 1:3200 | 1:3200 | 1:3200 | 1:3200 |
Mice were treated with either rapamycin or vehicle starting on the day of immunization (group 1; n = 8 in the rapamycin group, n = 6 in the vehicle group) or 14 d after induction of anti-GBM GN (group 2; n = 8 per group). We evaluated the cytokine expression profiles and FoxP3 expression in the lymph node (LN), splenocyte proliferation, and the amount of mouse anti-rabbit IgG in the serum 21 (group 1) or 35 d (group 2) after induction of anti-GBM GN. In addition we provided the titer of the deposited rabbit IgG on the GBM. The cytokine expressions evaluated by real-time PCR are given as fold increase compared with healthy controls. Data are means ± SEM.
P < 0.05 versus the respective control group.