Figure 4.

Segment-specific staining for injury. (A and B). Co-immunofluorescence for THP and HSV1-tk shows normal THP and absent HSV1-tk in GCV-treated transgenic-negative kidneys (A). GCV-treated transgenic-positive kidneys revealed distorted THP expression with THP-rich casts and lack of co-localization with HSV1-tk (B). (C through J). IHC for nephron-specific markers. THP, Lotus tetragonolobus lectin (LTL), thiazide-sensitive co-transporter (TSC), aquaporin-2 (AQP2) represent markers specific for the TAL, proximal tubule, distal convoluted tubule, and collecting duct, respectively. Segment staining appeared intact in all tubular segments except in GCV-treated transgenic-positive kidneys stained for THP (D), which demonstrated tubular casts, tubular dilation, and flattened epithelium Magnification, ×630.