Figure 10.

Relocalization of cytochrome c, recovery and proliferation of tunicamycin-treated cells upon removal of the apoptotic induction. (A) ETNA^−/− cells were treated with 3 μg/ml tunicamycin (Tun, 72 and 96 h). In parallel, ETNA^−/− cells treated with 3 μg/ml tunicamycin were washed after 48 h of treatment and medium was replaced with complete medium without tunicamycin (Tun+wash). Cells were kept in these conditions for additional 24 h (72 h-washed), 48 h (96 h-washed), 96 h (120 h-washed), and 120 h (144 h-washed). (B) ETNA^−/− cells were treated with 3 μg/ml tunicamycin; after 48 h of treatment, cells were washed, and medium was replaced with medium deprived of glucose and serum and were supplied with Mpyr, without tunicamycin (Tun+wash, no glycolysis). Cells were kept in these conditions for additional 24 h (72 h-washed-no glyc) and 48 h (96 h-washed-no glyc). Under these conditions, cells appeared shrunk and most of them were detached. After additional 96 h, cells were completely detached (not shown). Detection by immunofluorescence of cytochrome c localization (green) and nuclear DAPI staining (blue) is shown.