Figure 4.

Both ETNA^−/− and C9DNETNA cells are metabolically active after prolonged apoptotic treatment as seen by ATP content and MTS assay. (A) ATP production in ETNA^−/− and C9DNETNA cells treated with 3 μg/ml tunicamycin (Tun) or 20 μM actinomycin D (ActD) for the times indicated. A.U., arbitrary units. The ATP content has not been normalized for the amount of protein, but it has been evaluated after seeding and treating the same number of cells for each condition. (B) Reducing equivalent production measured by MTS-formazan assay in ETNA^−/− and C9DNETNA cells treated with tunicamycin (Tun) or actinomycin D (ActD) for the times indicated. Values represent the mean ± SD of four experiments.