Figure 9.

ROS production increases in absence of glycolysis. ETNA^−/−, C9DNETNA ETNA^−/−C2, C9DNETNA-C6, and Apaf1^−/− MEFs untreated (Ctrl), or treated for 48 h with tunicamycin (Tun), were cultured in complete medium (black line) or were deprived of glucose and serum and were supplied with Mpyr (gray shadow) 2 h before ROS measurement by FACS analysis. One hour before FACS analysis, cells were incubated with the ROS-sensitive probe DCF (30 μM). As negative control, cells cultured in complete medium were not incubated with DCF (red line).