Figure 1.

Synthetic growth defect between drs2Δ and gga alleles suggests that Drs2 acts in AP-1 pathways. (A) Tetrads derived from a drs2Δ cross with gga1Δgga2Δ. Circles indicate drs2Δ gga1Δgga2Δ triple mutants, and boxes are drs2Δ gga2Δ double mutants (tetrads, 1–4; spores, A–D). The unmarked WT and single mutant progeny grow equally well. (B) Growth of drs2Δ apl4Δ (γ-adaptin) and drs2Δ gga1Δ gga2Δ strains. Tenfold serial dilutions of strains BY4742 (WT), ZHY615M2D (drs2Δ), BY4742 YPR029C (apl4Δ), KLY691 (drs2Δ apl4Δ), KLY751 (gga1Δ gga2Δ), and KLY741 (drs2Δ gga1Δ gga2Δ) were spotted onto YPD plates and grown at 30 and 23°C.