Figure 3.

Loss of AP-1 perturbs Drs2p trafficking but not its steady-state TGN localization. (A) Colocalization of GFP-Drs2 and Sec7-DsRed at the TGN of WT (KLY1101) and AP-1–deficient (apl2Δ) cells (KLY351). Some of the cells are outlined for clarity. (B) Disruption of AP-1 subunit genes causes missorting of GFP-Drs2p to the plasma membrane, where accumulation was induced by blocking endocytosis with latrunculin A (Lat A) at room temperature for 30 min. WT (BY4741) and AP-1 mutant cells (BY4741 derivatives) were treated with Lat A and imaged as previously described (Liu et al., 2007).