Table 3.
Cysteine to serine mutations in the C-terminal domain of OOC-5 disrupt the rescuing ability of an ooc-5 transgene
| Transgenea | Worms holding embryos (n)b | Worms laying embryos (n)c | Embryonic viabilityd (%) |
|---|---|---|---|
| ooc-5 (it145); ooc-5 (+) | |||
| Line 1 | 4 | 15 | 72.04 |
| Line 2 | 0 | 17 | 20.90 |
| ooc-5 (it145); ooc-5 (C287S,C329S) | |||
| Line 1 | 17 | 20 | 5.11 |
| Line 2 | 10 | 10 | 3.26 |
| ooc-5 (it145) (no transgene) | |||
| Line 1 | 10 | 8 | 0.45 |
| Line 2 | 18 | 7 | 0.75 |
a Worms containing the wild-type ooc-5 transgene, the mutant ooc-5(C287S,C329S) transgene, or no transgene as indicated, were tested for their ability to rescue worms homozygous for the ooc-5(it145) mutation (see Materials and Methods). Because ooc-5 is a maternal effect lethal mutation, homozygous ooc-5 worms are viable, but produce virtually all dead embryos unless rescued by the transgene. Some homozygous worms also hold the majority of their eggs instead of laying them on the plate.
b Worms that laid fewer than 20 embryos.
c Worms that laid at least 20 embryos.
d L4 worms were picked to individual plates and removed after 48 h. Viability was calculated 24 h later from the number of larvae hatched divided by the total number of larvae and unhatched embryos. Viability was only scored from those worms laying at least 20 embryos.