Figure 7.

Developmental gene expression in vmp1⁻ mutant. (A) WT and mutant cells were deposited in nitrocellulose filters for development and photographs of a representative experiment were taken after 30 h. No aggregation was observed in the mutant. (B) RNA was isolated form cells developed on nitrocellulose filters for the indicated times, transferred to nylon membranes and hybridized to radioactive probes. The same blot was stripped and hybridized several times. The expression of the lectin Discoidin, the adenylyl cyclase ACA, and the cAMP receptor Car 1 was greatly reduced. The expression of P-type ATPase (PatA), which is not developmentally regulated, was used as a control. The staining of ribosomal RNAs by ethidium bromide is shown at the bottom.