Figure 3.

Normal glycoprotein metabolism in A4 cells. (A) The synthesis and turnover of total glycoproteins (captured using immobilized concanavalin-A) was assessed in A4 and N2a cells using pulse-chase analysis. Pulse labeling with [³⁵S]methionine was for 15 min, followed by chase for the indicated times (in hours). The positions of molecular weight markers (in kDa) are indicated on the left. (B) Synthesis and turnover of VSVG-GFP and ETBR-GFP in A4 and parental N2a cells was followed by pulse-chase analysis as in Figure 2A. Immunoprecipitation was with anti-GFP antibody. (C) Representative fluorescence images of A4 and N2a cells transfected with VSVG-GFP and ETBR-GFP.