Table 2.

Syb2 mutants reduce stability of SNARE complex dimers but not monomers

(A) Dimer stability: wild-type and syb2 mutant SNARE complexes were purified by size exclusion and analyzed by MALLS or analytical ultracentrifugation. [NaCl] at which SNARE complexes are 50% monomeric is taken from Figure 4c. (B) Monomer stability: SNARE complexes were subjected to differential scanning calorimetry to obtain melting point temperatures (Figure 3e). ΔH is based on integration of the melting point transition, whereas ΔH_VH is based on peak width and is independent of protein concentration.