Figure 2.

Cellular levels of proteins important for cell wall 1,6-β-glucan synthesis are decreased by the rot1-2 mutation. YM27 (ROT1 KRE5-HA), YM28 (rot1-2 KRE5-HA), YM41 (ROT1 HA-KRE6), YM42 (rot1-2 HA-KRE6), YM84 (ROT1 BIG1-HA), and YM85 (rot1-2 BIG1-HA) were incubated in YPD at 23 or 30°C or shifted from 23 to 37°C for the indicated times. The cell lysates were subjected to Western blotting using anti-HA, anti-Rot1, and anti-Sec61. The cellular level of Sec61 was not affected by the rot1-2 mutation and served as a loading control.