Table 3.

Rot1-depending proteins examined in this study

Protein	Property	Localization	Cellular function	Destabilization by rot1-2	Physical interaction with Rot1	Evolutional conservation
Kre5	Soluble protein	ER	1,6-β-glucan synthesis	Smalla	Yes	Conserved?b
Kre6	Type II m.p.c	ER∼Golgi	1,6-β-glucan synthesis	Higha	Yes	Yeast specific
Big1	Type I m.p.	ER	1,6-β-glucan synthesis	Mediumd	Yes	Yeast specific
Atg22	m.p. (10 TMs)e	Vacuole	Amino acid permease?	Mediumf	Not determined	Yeast, bacteria
Drs2	m.p. (8 TMs)e	Golgi∼PMg	Phospholipid traslocation?	Mediuma	Yes	Conservedh
Gup1	m.p. (11 TMs)e	ER	GPI anchor remodeling	Mediuma	Not detected	Conservedh

^a Estimated by [³⁵S]Met/Cys pulse-chase experiments.

^b Function(s) of Kre5 may be different from that of UGGT (Levinson et al., 2002).

^c Membrane protein.

^d Estimated by cycloheximide-chase experiments.

^e Number of the transmembrane regions predicted by SOSUI (http://bp.nuap.nagoya-u.ac.jp/sosui/).

^f Estimated by Western blotting.

^g Plasma membrane.

^h Homologous protein(s) is registered in SWISS-PLOT, but its function is unknown.