Figure 2.

FLAG-UbcH5C colocalizes with GFP-Cbl and EGF in the plasma membrane and endosomes. CHO cells were transiently transfected with EGFR, GFP-Cbl, and the indicated FLAG-E2 plasmids and stimulated with Alexa 647-EGF for 2 or 15 min. In D, the mutant EGFR(Y1045F) was expressed instead of the wild-type receptor. The cells were stained with the anti-FLAG antibody. Arrows in B and C indicate representative colocalizing structures in the plasma membrane and endosomes, respectively. Bar, 20 μm.