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. 2008 Aug;19(8):3290–3298. doi: 10.1091/mbc.E07-12-1292

Figure 2.

Figure 2.

Atg8 controls the size of the autophagosome. (A and B) Lower amounts of Atg8 reduce the sizes of autophagosomes. Yeast cells were grown in rich medium to midlog phase and then starved in nitrogen-starvation medium for 4 h, fixed in potassium permanganate, and processed for EM. (A) Representative EM images from pep4Δ strains. Autophagic bodies accumulated in A8-1, A8-2, A8-3, and wild-type background strains. No autophagic bodies were found in atg8Δ background cells. Scale bar, 1 μm. (B) Quantification of autophagic body size. The average radii of cross-sections of autophagic bodies are shown; Error bar, SEM; n > 200. Autophagic bodies in the A8-1, A8-2, and A8-3 strains were significantly smaller than those of the wild-type (WT) background. (C) Atg8 level does not limit the number of autophagosomes produced. Yeast cells starved for 2 and 4 h were collected and processed for EM. The average numbers of autophagic bodies per vacuole section are shown. Error bar, SEM; n > 100. Similar numbers of autophagic bodies were observed in strains with different amounts of Atg8.