Figure 4.

Arf4, but not Arf1, remains associated with the ERGIC after BFA treatment. (A) COS1 cells cotransfected with plasmids encoding Arf1-GFP and Arf4-mCherry were imaged continuously for 11 min after BFA addition. Panels show single channel images at the indicated time points. Representative of at least three experiments. Bar, 10 μm. (B) COS1 cells cotransfected with plasmids encoding Arf1-GFP and Arf4-mCherry were imaged continuously for 2 min after BFA addition and an additional 9 min after BFA washout as described in Materials and Methods. Panels show single channel images at the indicated times. Representative of at least three experiments. Bar, 10 μm.