Figure 6.

Exo1 causes GBF1 to concentrate on the Golgi complex and the ERGIC. (A) NRK cells were treated with carrier DMSO or 100 μM Exo1 for 90 s, fixed, and processed for IF by using anti-GBF1 monoclonal antibody as described in Materials and Methods. Images were acquired and processed identically. Bar, 10 μm. (B) NRK-GFP-GBF1 cells were treated with carrier DMSO or 100 μM Exo1 and imaged for 10 min. Images correspond to single frames at the indicated time points. Images representative of at least four experiments in NRK cells. Bar, 10 μm. (C) Quantification of GFP-GBF1 relative signal intensities (percentage of initial value) at the Golgi and peripheral puncta membranes after 1 min of treatment with Exo1. Values correspond to averages with SE obtained from a minimum of 25 cells from at least four different sets of experiments.