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Phosphorylation of maskin restores centrosome function. (A and B) Quantification of centrosome activity in the sequential assay, expressed as the number of asters found in 50 randomly chosen microscope fields. Centrosomes were incubated with recombinant maskin phosphorylation mutants (A), or recombinant maskin in the presence or absence of ATP (B) as indicated below the graphs. Graphs represent three independent experiments (indicated by circles).
