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Simultaneous observation of FSM and microtubules. (A) Two color live imaging of YFP-Psy1 (FSM) and CFP-Atb2 (microtubules) in wild-type strain TN344. In merged images, YFP-Psy1 (green) and CFP-Atb2 (red) are shown. Frames were taken every 2 min. Bar, 10 μm. (B) Kinetics of FSM expansion and spindle extension during meiosis II. The contour length of one out of four FSMs (green line) and one of two spindle microtubules (red line) was measured by Aquacosmos software and plotted.
