Figure 5.

Behavior of GFP-tagged Psy1 during sporulation in the spo3 mutant. spo3Δ strain TN240 which expresses GFP-Psy1, was cultured on sporulation medium (SSA) for 16 h, and stained with Hoechst 33342 to allow simultaneous observation of chromatin by time-lapse fluorescence microscopy. Frames were taken every 2 min. In merged images, GFP-Psy1 (green) and Hoechst 33342 (magenta) are shown. (A) Class I mutant: four aggregates of GFP-Psy1 are formed near nuclei. (B) Class II mutant: four nucleated prespores are formed but they are remarkably small. Bar, 10 μm.