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. 2008 Aug 13;3(8):e2921. doi: 10.1371/journal.pone.0002921

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Brookes et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Short term cell lines specific for p23 were generated using PBMC recovered 28 days after vaccination and specificity demonstrated by flow cytometry. Gating on IFN-γ detection, both CD4 and CD8 responses were observed for one subject (volunteer 1) while for the other subject (volunteer 2) an exclusive CD8 response was identified. B. Volunteer 2 also showed CD4 and CD8 activation by CD69 expression after stimulation with pooled Ag85A peptides 28 days after vaccination.