Figure 2. Flow cytometric analysis of polyp infitrate.

(A) FACS analyses of pro-inflammatory cells. (a) Exemplar FACS analyses of mononuclear cells prepared from the intestine of (a&c) a 5 month-old APC^Δ468 mouse and (b&d) an age matched wt C57BL/6J mouse; note increases in the frequencies of (a) CD11b⁺Gr1⁺ and (c) CD11b⁺F4/80⁺ myeloid type cells in the polyposis intestine, compared to wt tissue (b&d respectively). (B) Summary of FACS analysis of MNCs from wt (black bars) or APC^Δ468 (open bars) intestine, showing mean frequencies and absolute numbers of cells with SEM values; n = 6 for APC^Δ468, n = 3 for wt control mice, per age group.